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Наименование:

SINGLE-CELL GENE-EXPRESSION ANALYSIS. QUANTITATIVE RT-PCR

Автор:

мягкая обложка
232 страниц
2009 год

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The sensitivity of the assays now in common use limits the resolution with which we can follow the activity of genetic-regulatory networks. We now understand the general schemes by which transcriptional networks regulate cellular differentiation and morphogenesis. This thesis describes two projects aimed at refining an established gene-profiling method, quantitative RT-PCR, so that it can be used to profile transcriptional-network states cross-sectionally within developing cell populations at single-cell resolution. With stem-cell therapies on the horizon, the reverse engineering of developmental programs promises to become a task of great practical significance. The problem of development has long been one of the key issues in biology. These genetic circuits function as complex state machines which, over the course of development, undergo sequenced transitions that bring cells to specific end states. A variety of different gene-expression assays can be used to follow these transitions. Two advanced qRT-PCR protocols were developed to support these projects, one based on microfluidic digital PCR, the other based on multiplexed pre-amplification PCR.

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Human Molecular Genetics 8: 1839-1846 Le Guyader, H. 1998. Etienne Geoffroy Saint-Hilaire (1772-1844): un naturalist visionnaire. Belin, Paris Le Mouellic, H. et al. 1992. Homeosis in the mouse induced by a null mutation in the HOX-3.1 gene. Cell 69: 251-264 Le Roith, D. et al. 2001. What is the role of circulating IGF? Trends in Endocrinology and Metabolism 12: 48-52 Lee, C.-K. et al. 1999. Gene expression profile of aging and its retardation by caloric restriction. Science 285: 1390-1393 Lee, H.W. et al. 1998. Essential role of mouse telomerase in highly proliferative organs. Nature 392: 569-574 Lee, P.A. and S.R Witchel. 1997. The influence of estrogen on growth. Current opinion in pediatrics 9: 431-436 Lenz, W. 1962. Thalidomide and congenital abnormalities. Lancet 1: 45 Leroi, A.M. 2001. Molecular signals versus the loi de balancement. Trends in Ecology and Evolution. 16: 24-29 Leroi, A.M. et al. 2003. Cancer selection. Nature Cancer Reviews 3: 226-231 Lettice, L.A. et al. 2002. Disruption of a long-range cis-acting regulator for Shh causes preaxial Polydactyly ...»

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SINGLE-CELL GENE-EXPRESSION ANALYSIS. QUANTITATIVE RT-PCR Harry N. http://www.booksiti.net.ru/books/14692650

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